کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10410449 | 894437 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A hydrogen peroxide biosensor based on nano-Au/PAMAM dendrimer/cystamine modified gold electrode
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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![عکس صفحه اول مقاله: A hydrogen peroxide biosensor based on nano-Au/PAMAM dendrimer/cystamine modified gold electrode A hydrogen peroxide biosensor based on nano-Au/PAMAM dendrimer/cystamine modified gold electrode](/preview/png/10410449.png)
چکیده انگلیسی
Horseradish peroxidase (HRP) to a nano-Au monolayer, which was supported by PAMAM dendrimer/cystamine modified gold electrode. The high affinity of PAMAM dendrimer for nano-Au with its amine groups was used to realize the role of nano-Au as an intermediator to retain the high bioactivity of HRP. A hydrogen peroxide biosensor was fabricated based on the effective immobilization of the high bioactivity of HRP. In the presence of the hydroquinone mediator in the solution, the immobilized HRP exhibited excellent electrocatalytical response to the reduction of H2O2. The effects of various experimental variables were optimized. The resulting biosensor showed a linear response to H2O2 over a concentration range from 1 à 10â5 to 2.5 à 10â3 mol Lâ1 with a sensitivity of 0.53 A L molâ1 cmâ2 and a detection limit of 2.0 μmol Lâ1 based on a signal-to-noise ratio of 3. The apparent Michaelis-Menten constant (Kmapp) of the biosensor was evaluated to be 0.52 mmol Lâ1. The biosensor exhibited high sensitivity and good stability and reproducibility. Performance of the biosensor was evaluated with respect to possible interferences and the application to real sample analysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 106, Issue 1, 29 April 2005, Pages 394-400
Journal: Sensors and Actuators B: Chemical - Volume 106, Issue 1, 29 April 2005, Pages 394-400
نویسندگان
Zhi-Min Liu, Yu Yang, Hua Wang, Yan-Li Liu, Guo-Li Shen, Ru-Qin Yu,