کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10410811 894507 2006 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fabrication of patterned micromuscles with high activity for powering biohybrid microdevices
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Fabrication of patterned micromuscles with high activity for powering biohybrid microdevices
چکیده انگلیسی
In this paper, we report a microfabrication approach to patterned three-dimensional growth of muscle cells for powering biohybrid microdevices. The microwells confined by adhesion-resistant polyethylene glycol (PEG) microstructures were patterned on SiO2-based substrate with controllable surface topography for muscle cell culturing. The morphology and motility of neonatal rat cardiac myocytes patterned within microwells were analyzed with different topographical heights of the PEG barrier. It was found that isolated aggregates of cardiac muscles showed various beating activities depending on the morphology and the number of cells. Furthermore, three-dimensionally grown cardiac muscle cells mediated by a higher physical barrier of PEG microstructures generated higher contraction force with faster beating frequency than those of cells attached to the collagen-coated surface on the culture dish (control), suggesting that control over surface topography of microwells for cell growth would be potentially useful in engineering cell motors. Thus the technique presented here would provide a valuable platform for optimizing the activity and functions of patterned muscle cells in building up integrated bioactuated microdevices.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 117, Issue 2, 12 October 2006, Pages 391-400
نویسندگان
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