کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10410820 | 894507 | 2006 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel real-time immunoassay utilizing an electro-immunosensing microchip and gold nanoparticles for signal enhancement
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A novel real-time immunoassay utilizing an electro-immunosensing microchip and gold nanoparticles for signal enhancement A novel real-time immunoassay utilizing an electro-immunosensing microchip and gold nanoparticles for signal enhancement](/preview/png/10410820.png)
چکیده انگلیسی
This study focused on the development of an electro-immunosensing (EIS) microchip for real-time measurement of antibody-antigen recognition in immunoassay. Instead of the enzyme being conjugated with a secondary antibody for detection, the process of electro-immunoassay was found to be relatively simpler compared to the conventional enzyme-linked immunosorbent assay (ELISA). This novel testing method was designed using the wave impedance theory to measure the antibody-antigen recognition. Based on the phase variety in the frequency domain, the detection limit of protein A was 1Â ng/mL. It was found that the antibody-antigen recognition has an obvious peak of the phase angle near 1.61Â GHz. The EIS chip had higher sensitivity and a shorter assay time than the ELISA. The sensitivity of the immunoassay on the EIS chip was 100-fold higher than that of conventional enzyme-linked immunosorbent assay (ELISA). Using antibodies labelled with 13Â nm gold nanoparticles (ANPs), the detection sensitivity of protein A was increased to 0.1Â ng/mL. In addition, the EIS chip was capable of detecting the kinematics of antibody-antigen binding.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 117, Issue 2, 12 October 2006, Pages 451-456
Journal: Sensors and Actuators B: Chemical - Volume 117, Issue 2, 12 October 2006, Pages 451-456
نویسندگان
Min Li, Yu-Cheng Lin, Kai-Chun Su, Yu-Tsung Wang, Tsung Chain Chang, Hong-Ping Lin,