کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10411076 | 894540 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In situ monitoring of polysaccharide-polynucleotide interaction using a schizophyllan-immobilized QCM device
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
Schizophyllan belongs to the β-(1 â 3)-d-glucan family and is well known as a triple helix polysaccharide. It is already known that the single chain of schizophyllan (s-SPG) can specifically form a complex with particular polynucleotides. As an application of this recognition capability, a novel schizophyllan derivative connected with a disulfide bond through a long methylene spacer was synthesized, and its interaction with polynucleotides was directly monitored with a self-assembled monolayer on QCM. The gold-coated QCM electrode was covered in ca. 60% as a monolayer of s-SPG. The QCM resonator showed sensitive mass increases for poly(C) and poly(U) in the presence of K+ ion, which are consistent with our previous results. The present results indicate that the schizophyllan-immobilized QCM resonator can be a new sensing device for biomolecules.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 105, Issue 2, 28 March 2005, Pages 490-494
Journal: Sensors and Actuators B: Chemical - Volume 105, Issue 2, 28 March 2005, Pages 490-494
نویسندگان
Do-Hyeon Yang, Ah-Hyun Bae, Kazuya Koumoto, Seung-Woo Lee, Kazuo Sakurai, Seiji Shinkai,