کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10412753 895152 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
GaAs/AlGaAs heterostructure based photonic biosensor for rapid detection of Escherichia coli in phosphate buffered saline solution
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
GaAs/AlGaAs heterostructure based photonic biosensor for rapid detection of Escherichia coli in phosphate buffered saline solution
چکیده انگلیسی
We have investigated photonic biosensing of bacteria based on photoluminescence (PL) of GaAs/AlGaAs heterostructures. The method takes advantage of the GaAs PL sensitivity to the perturbation of the semiconductor near-surface electric field induced by the charge of bacteria immobilized in its vicinity. Maintaining the balance between device sensitivity and stability in the biosensing (aqueous) environment is one of the key parameters allowing successful biosensing. To immobilize bacteria, we have employed a network of biotinylated antibodies interfaced with biotinylated polyethylene glycol thiols through the link provided by neutravidin. Post-processing of thiolated samples in ammonium sulfide was applied to increase the stability of the biosensing architectures while allowing biosensing at an attractive level of detection. Escherichia coli was detected in phosphate buffered saline solutions at 103 CFU/mL; however, it appears that even greater sensitivity levels are feasible with this technique.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 207, Part A, February 2015, Pages 556-562
نویسندگان
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