کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10429177 909698 2005 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Crocin Synthesis Mechanism in Crocus sativus
موضوعات مرتبط
مهندسی و علوم پایه سایر رشته های مهندسی مهندسی (عمومی)
پیش نمایش صفحه اول مقاله
Crocin Synthesis Mechanism in Crocus sativus
چکیده انگلیسی
Saffron (Crocus sativus) cells can synthesize crocin, crocetin digentiobiosyl ester, in suspension cultures. The crocin family biosynthesis mechanism was studied using high pressure liquid chromatography (HPLC) to determinate the glucosyltransferase activity and to develop a method for synthesizing medicine from saffron cells. Previous studies indicated that two glucosyltransferases might be involved in the formation of crocetin glucosyl- and gentiobiosyl-esters. GTase1 formed an ester bond between crocetin carboxyl groups and glucose moieties while GTase2 catalyzed the formation of glucosidic bonds with glucosyl ester groups at both ends of the molecule. These enzymes can catalyze the formation of crocetin glucosides in vitro. GTase1 activity is higher during the first four days of crocin glucosides biosynthesis, but decreases after four days. The formation and accumulation of crocin increase during the first six days and stabilized on the eighth day.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Tsinghua Science & Technology - Volume 10, Issue 5, October 2005, Pages 567-572
نویسندگان
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