کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10532551 | 961609 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A chronocoulometric aptasensor based on gold nanoparticles as a signal amplification strategy for detection of thrombin
ترجمه فارسی عنوان
یک سنجش سنجی کرونوکولومتریک مبتنی بر نانوذرات طلا به عنوان یک استراتژی تقویت سیگنال برای تشخیص ترومبین
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A sensitive chronocoulometric aptasensor for the detection of thrombin has been developed based on gold nanoparticle amplification. The functional gold nanoparticles, loaded with link DNA (LDNA) and report DNA (RDNA), were immobilized on an electrode by thrombin aptamers performing as a recognition element and capture probe. LDNA was complementary to the thrombin aptamers and RDNA was noncomplementary, but could combine with [Ru(NH3)6]3+ (RuHex) cations. Electrochemical signals obtained by RuHex that bound quantitatively to the negatively charged phosphate backbone of DNA via electrostatic interactions were measured by chronocoulometry. In the presence of thrombin, the combination of thrombin and thrombin aptamers and the release of the functional gold nanoparticles could induce a significant decrease in chronocoulometric signal. The incorporation of gold nanoparticles in the chronocoulometric aptasensor significantly enhanced the sensitivity. The performance of the aptasensor was further increased by the optimization of the surface density of aptamers. Under optimum conditions, the chronocoulometric aptasensor exhibited a wide linear response range of 0.1-18.5Â nM with a detection limit of 30Â pM. The results demonstrated that this nanoparticle-based amplification strategy offers a simple and effective approach to detect thrombin.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 441, Issue 2, 15 October 2013, Pages 95-100
Journal: Analytical Biochemistry - Volume 441, Issue 2, 15 October 2013, Pages 95-100
نویسندگان
Xiao Xia Jiao, Jing Rong Chen, Xi Yuan Zhang, Hong Qun Luo, Nian Bing Li,