کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10532582 | 961630 | 2013 | 29 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Correction factors for membrane protein molecular weight readouts on sodium dodecyl sulfate-polyacrylamide gel electrophoresis
ترجمه فارسی عنوان
فاکتورهای اصلاح شده برای اندازه گیری وزن مولکول پروتئین غشایی بر روی الکتروفورز ژل دوده سولفات سدیم پلی آکریل آمید
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Membrane proteins are known to migrate anomalously on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to relative molecular mass (Mr) values larger or smaller than formula molecular weight. We constructed a database from literature Mr values reported for 168 nonredundant helical membrane proteins with structures determined to high resolution and found that more than three-quarters of them exhibit this behavior on gels calibrated with commercial standards. Further analysis of the database indicated that the direction of anomalous migration is not a consequence of membrane protein net charge or hydrophobicity. Plots of observed versus formula Mr values showed that membrane proteins migrating slower than expected read out at 1.13 Ã Mr, whereas those that migrate faster than expected read out at 0.82 Ã Mr (R2 â¼Â 0.98, P < 0.0001). These robust trends imply that division of the Mr readouts of slower or faster migrating analytes by 1.13 or 0.82, respectively, should enhance SDS-PAGE accuracy. Applying this correction procedure to SDS-PAGE readouts of four fast-migrating helical transmembrane (TM) proteins significantly reduced Mr errors from approximately 20% to 8% (P < 0.0001). Our results suggest that hydrophobic standards for SDS-PAGE would significantly improve the performance of the technique applied to membrane proteins.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 434, Issue 1, 1 March 2013, Pages 67-72
Journal: Analytical Biochemistry - Volume 434, Issue 1, 1 March 2013, Pages 67-72
نویسندگان
Arianna Rath, Charles M. Deber,