کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10532595 961630 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of total plasma hydroperoxides using a diphenyl-1-pyrenylphosphine fluorescent probe
ترجمه فارسی عنوان
تعیین هیدروپراکسیداسیون کل پلاسما با استفاده از پروب فلورسنت دیفنیل-1-پیرانیل فسفین
کلمات کلیدی
استرس اکسیداتیو، هیدروپراکسید، پلاسما، دیفنیل-1-پیرینیل فسفین،
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Plasma hydroperoxides (HPs) are widely accepted to be good indicators of oxidative stress. By means of the method proposed here, which uses diphenyl-1-pyrenylphosphine (DPPP) as a fluorescent probe, all types of plasma HP were determined. The limits of detection and quantification of the method were 0.08 and 0.25 nmol of cumene hydroperoxide (CHP) equivalents in 40 μl of plasma, respectively. The method is satisfactory in terms of precision (5.3% for 14.5 μM CHP eq., n = 8), and the recoveries were 91% and 92% after standard additions of 26 and 52 μM CHP, respectively. The selectivity of the proposed method is higher than 96%. Moreover, optimization of the reaction conditions and the addition of ethylenediaminetetraacetic acid (EDTA) disodium salt and 2,6-di-tert-butyl-4-methylphenol (BHT) prevented the formation of HP artifacts during the analysis. Therefore, the proposed method is useful for simple and quantitative determination of total plasma HPs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 434, Issue 1, 1 March 2013, Pages 172-177
نویسندگان
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