کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10532623 961673 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A simple set-and-mix assay for screening of protein kinase inhibitors in cell lysates
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A simple set-and-mix assay for screening of protein kinase inhibitors in cell lysates
چکیده انگلیسی
Here we developed a simple set-and-mix assay to perform high-throughput screening of protein kinase A (PKA) inhibitors from the LOPAC 1280 compound library. This assay is based on the color change of gold nanoparticles on aggregation induced by a cationic substrate peptide as coagulant. In spite of the simplicity of this assay system, this assay can be applied to drug screening based on cellular kinases. We successfully found several highly active inhibitors, including compounds that have not been reported before.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 418, Issue 1, 1 November 2011, Pages 44-49
نویسندگان
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