کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10532769 961712 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Modified nano-TiO2 coupled with fluorescence spectroscopy for the separation/analysis of l-tryptophan
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Modified nano-TiO2 coupled with fluorescence spectroscopy for the separation/analysis of l-tryptophan
چکیده انگلیسی
A novel adsorbent of carboxymethyl-β-cyclodextrin modified nanometer TiO2 (CM-β-CD/TiO2) was prepared and used as a solid-phase extraction (SPE) material coupled to fluorescence spectroscopy determination of l-tryptophan (l-Trp) in biological samples. The experimental conditions for modified nanometer TiO2 separation/preconcentration of l-Trp were optimized. The adsorption capacity of CM-β-CD/TiO2 for l-Trp was 75.2 μg/g. The linear range, detection limit (DL), and the relative standard deviation (RSD) were 0.10-1.20 μg/mL, 18.8 ng/mL, and 0.67% (n = 3, 1.0 μg/mL), respectively, with a preconcentration factor of 10. The developed method was applied to determination of l-Trp in real samples and the recoveries were found to be in the range of 99.2-100.3%. For validation, a comparison material of NIC-140686 sample was analyzed and the determined value was in good agreement with the certified value.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 401, Issue 2, 15 June 2010, Pages 260-265
نویسندگان
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