کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10532828 961725 2013 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Watch out for reporter gene assays with Renilla luciferase and paclitaxel
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Watch out for reporter gene assays with Renilla luciferase and paclitaxel
چکیده انگلیسی
Luminescence-based reporter gene assays are widely used in biochemistry. Signals from reporter genes (e.g., firefly luminescence) are usually normalized to signals from constantly luminescing luciferases such as Renilla luciferase. This normalization step can be performed by modern luminometry devices automatically providing final results. Here we demonstrate paclitaxel to strikingly enhance Renilla luminescence, thereby potentially flawing results from reporter gene assays. In consequence, these data advocate for careful examination of raw data and militate against automatic data processing.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 437, Issue 2, 15 June 2013, Pages 109-110
نویسندگان
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