کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10532848 961744 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Electron transfer mediated by membrane-bound d-fructose dehydrogenase adsorbed at an oil/water interface
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Electron transfer mediated by membrane-bound d-fructose dehydrogenase adsorbed at an oil/water interface
چکیده انگلیسی
The catalytic activity of a membrane-bound enzyme, d-fructose dehydrogenase (FDH), at the polarized oil/water (O/W) interface was studied. Multisweep cyclic voltammetry and ac voltammetry were carried out to show the irreversible adsorption of FDH at the interface. Using the thusly prepared FDH-adsorbed O/W interface, clear steady-state catalytic current was observed in amperometry and cyclic voltammetry, where 1,1′-dimethylferrocenium ion (DiMFc+, electron acceptor) and d-fructose (substrate) were added to the O and W phases, respectively. The observed catalytic current was then analyzed by using two mechanisms. In mechanism (A), the heme c site of FDH, where DiMFc+ is reduced, was assumed to be located in the O-phase side of the interface. The intramolecular electron transfer in FDH should be affected by the Galvani potential difference of the interface (ΔOWϕ). However, the theoretical equations derived for the catalytic current could not reproduce the experimental data. In mechanism (B), the heme c site was assumed to be in the W-phase side. In this case, ΔOWϕ should affect the interfacial distribution of DiMFc+. This mechanism could reproduce well the observed potential dependence of the catalytic current.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 417, Issue 1, 1 October 2011, Pages 129-135
نویسندگان
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