کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10533014 | 961836 | 2005 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expanding the range of free calcium regulation in biological solutions
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
Many biological systems use ethylene glycol bis (β-aminoethylether)-N,N,Nâ²,Nâ²-tetraacetic acid (EGTA) to regulate the free calcium concentration ([Ca2+]free) in the presence of physiological levels of free Mg2+ ([Mg2+]free). Frequently, it is necessary to work at [Ca2+]free beyond EGTA's buffering capabilities. Therefore, we have developed methods to extend the buffering range by adding nitrilotriacetic acid (NTA) to solutions containing EGTA. This extension results from NTA having a lower KdCaâ² than EGTA. Such equilibria are solved by pCa Calculator, a computer program designed to aid in the study of Ca2+-dependent physiological processes while accounting for the effects of pH, temperature, and ionic strength. With multiple chelators and pH buffers from which to choose, pCa Calculator calculates the total concentration of each species required to achieve specified free concentrations of Ca2+, ATP, and Mg2+. The program is intuitive, user-friendly, and flexible enough to fix or vary the [Mg-ATP2â] and ionic strength. Moreover, it can account for increases in experimental volume from calcium addition. A comparative analysis is reported for testing solutions in the presence and absence of NTA by measuring the calcium binding affinity of fluorescent cardiac troponin C. These findings demonstrate that EGTA, when used in conjunction with NTA, improves and expands the regulation of free calcium in solution.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 347, Issue 2, 15 December 2005, Pages 303-315
Journal: Analytical Biochemistry - Volume 347, Issue 2, 15 December 2005, Pages 303-315
نویسندگان
David Dweck, Avelino Jr., James D. Potter,