کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10533128 | 961849 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Spectrofluorimetric determination of heparin using a tetracycline-europium probe
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Spectrofluorimetric determination of heparin using a tetracycline-europium probe Spectrofluorimetric determination of heparin using a tetracycline-europium probe](/preview/png/10533128.png)
چکیده انگلیسی
A new spectrofluorimetric method was developed for the determination of trace amounts of heparin (Hep). Using tetracycline (TC)-europium ion (Eu3+) as a fluorescent probe, in the buffer solution at pH 8.8, Hep can remarkably enhance the fluorescence intensity of the TC-Eu3+ complex at λ = 612 nm and the enhanced fluorescence intensity of Eu3+ is in proportion to the concentration of Hep. Optimal conditions for the determination of Hep were also investigated. The linear range and detection limit for the determination of Hep were 0.02 to 1.6 μg/ml and 4.45 ng/ml, respectively. This method is simple, practical, and relatively free of interference from coexisting substances, and it can be applied successfully to assess Hep in biological samples. By the Rosenthal graphic method, the association constant and binding numbers of Hep with the probe were 4.46 Ã 104 L/mol and 16.2, respectively. Moreover, the enhancement mechanism of the fluorescence intensity in the TC-Eu3+ system, the TC-Eu3+-Hep system, and the TC-Eu3+-Hep-CTMAB system is also discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 341, Issue 2, 15 June 2005, Pages 299-307
Journal: Analytical Biochemistry - Volume 341, Issue 2, 15 June 2005, Pages 299-307
نویسندگان
Xiaojing Zhu, Xiaolei Wang, Chongqiu Jiang,