کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10533158 961851 2005 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Detection of cruciform extrusion in DNA by temperature-gradient gel electrophoresis
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Detection of cruciform extrusion in DNA by temperature-gradient gel electrophoresis
چکیده انگلیسی
Repetitive sequences in DNA molecules, some of which are palindromic, tend to form stable cruciforms. These are frequently located in promoter regions of a specific operon and origin of replication. Temperature gradient gel electrophoresis can be used to distinguish among various supercoiled DNA topoisomers and to ascertain whether or not the cruciform motif has been extruded. In the current study, this technique is implemented for the first time to address the role of temperature in cruciform extrusion from plasmids.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 343, Issue 2, 15 August 2005, Pages 308-312
نویسندگان
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