کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10533214 | 961856 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
RecA-mediated multistrand formation for cloning PCR products into vectors: Simplified process for 5â²-rapid amplification of cDNA ends
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: RecA-mediated multistrand formation for cloning PCR products into vectors: Simplified process for 5â²-rapid amplification of cDNA ends RecA-mediated multistrand formation for cloning PCR products into vectors: Simplified process for 5â²-rapid amplification of cDNA ends](/preview/png/10533214.png)
چکیده انگلیسی
I have developed a novel rapid amplification of cDNA ends (RACE) technology that uses multistranded DNA formation mediated by the RecA protein. Multistranded DNA can readily be formed at the terminus of double-stranded DNA by a complementary single-stranded DNA in the presence of RecA and exonuclease I. The possibility of applying this finding to the direct cloning of a 5â²-RACE product onto a cDNA fragment, which does not require the use of restriction endonucleases, was explored. The results show that the terminal multistranded structure formed by the RecA-mediated reaction can be applied to RACE systems. Modifications to the RACE protocol to improve the effectiveness of the technique are also suggested.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 341, Issue 1, 1 June 2005, Pages 141-147
Journal: Analytical Biochemistry - Volume 341, Issue 1, 1 June 2005, Pages 141-147
نویسندگان
Yasushi Shigemori,