کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10533253 961860 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Gold nanoparticle aggregation for quantification of oligonucleotides: Optimization and increased dynamic range
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Gold nanoparticle aggregation for quantification of oligonucleotides: Optimization and increased dynamic range
چکیده انگلیسی
A variety of assays have been proposed to detect small quantities of nucleic acids at the point of care. One approach relies on target-induced aggregation of gold nanoparticles functionalized with oligonucleotide sequences complementary to adjacent regions on the targeted sequence. In the presence of the target sequence, the gold nanoparticles aggregate, producing an easily detectable shift in the optical scattering properties of the solution. The major limitations of this assay are that it requires heating and that long incubation times are needed to produce a result. This study aimed to optimize the assay conditions and optical readout, with the goals of eliminating the need for heating and reducing the time to result without sacrificing sensitivity or dynamic range. By optimizing assay conditions and measuring the spectrum of scattered light at the end point of incubation, we found that the assay is capable of producing quantifiable results at room temperature in 30 min with a linear dynamic range spanning 150 amol to 15 fmol of target. If changes in light scattering are measured dynamically during the incubation process, the linear range can be expanded 2-fold, spanning 50 amol to 500 fmol, while decreasing the time to result to 10 min.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 431, Issue 2, 15 December 2012, Pages 99-105
نویسندگان
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