کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10533253 | 961860 | 2012 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Gold nanoparticle aggregation for quantification of oligonucleotides: Optimization and increased dynamic range
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A variety of assays have been proposed to detect small quantities of nucleic acids at the point of care. One approach relies on target-induced aggregation of gold nanoparticles functionalized with oligonucleotide sequences complementary to adjacent regions on the targeted sequence. In the presence of the target sequence, the gold nanoparticles aggregate, producing an easily detectable shift in the optical scattering properties of the solution. The major limitations of this assay are that it requires heating and that long incubation times are needed to produce a result. This study aimed to optimize the assay conditions and optical readout, with the goals of eliminating the need for heating and reducing the time to result without sacrificing sensitivity or dynamic range. By optimizing assay conditions and measuring the spectrum of scattered light at the end point of incubation, we found that the assay is capable of producing quantifiable results at room temperature in 30Â min with a linear dynamic range spanning 150Â amol to 15Â fmol of target. If changes in light scattering are measured dynamically during the incubation process, the linear range can be expanded 2-fold, spanning 50Â amol to 500Â fmol, while decreasing the time to result to 10Â min.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 431, Issue 2, 15 December 2012, Pages 99-105
Journal: Analytical Biochemistry - Volume 431, Issue 2, 15 December 2012, Pages 99-105
نویسندگان
Michael S. Cordray, Matthew Amdahl, Rebecca R. Richards-Kortum,