کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10533737 | 961890 | 2012 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The effective time of centrifugation for the analysis of boundary spreading in sedimentation velocity experiments
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
This investigation establishes a likely order of magnitude for the zero-time correction factor governing the effective time of centrifugation that is pertinent in the analysis of boundary spreading in sedimentation velocity experiments. This correction is shown to be too small to unduly affect the magnitudes of sedimentation and diffusion coefficients deduced from the application of computer software incorporating the printout value of Ï2t and an effective position of the air-solution meniscus that is obtained as an additional parameter in the analysis involving nonlinear least-squares curve-fitting of sedimentation velocity distributions to the Lamm equation. Although this procedure slightly underestimates the actual meniscus position (ra), uncertainty about its exact location precludes adoption of the alternative approach with ra fixed and the correction to Ï2t regarded as the additional curve-fitting parameter.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 421, Issue 2, 15 February 2012, Pages 755-758
Journal: Analytical Biochemistry - Volume 421, Issue 2, 15 February 2012, Pages 755-758
نویسندگان
Tabot M.D. Besong, Stephen E. Harding, Donald J. Winzor,