کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10533938 | 961900 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Assay for the quantification of small-sized fragmented genomic DNA
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Assay for the quantification of small-sized fragmented genomic DNA Assay for the quantification of small-sized fragmented genomic DNA](/preview/png/10533938.png)
چکیده انگلیسی
This paper presents a simple assay for the direct quantification of small-sized (up to 1000 bp) fragmented DNA based on the differential separation between large- and small-sized DNA by polyethylene glycol precipitation. The assay can be applied to as low as 1 μg mlâ1 DNA and has a very low DNA detection limit (0.01 μg mlâ1 fragmented DNA), which can be further lowered at least 10-fold with anion exchange chromatography. The assay quantifies for the first time a more direct indicator of the extent of DNA damage than the usually assessed DNA nicks and base modifications, since small-sized fragmented DNA represents an unrepairable DNA damage of necrotic and apoptotic events that are related to normal and abnormal biological conditions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 339, Issue 2, 15 April 2005, Pages 223-230
Journal: Analytical Biochemistry - Volume 339, Issue 2, 15 April 2005, Pages 223-230
نویسندگان
Christos D. Georgiou, Nikolaos Patsoukis, Ioannis Papapostolou,