کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10534090 | 961904 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A high-throughput screen utilizing the fluorescence of riboflavin for identification of lumazine synthase inhibitors
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A high-throughput screen utilizing the fluorescence of riboflavin for identification of lumazine synthase inhibitors A high-throughput screen utilizing the fluorescence of riboflavin for identification of lumazine synthase inhibitors](/preview/png/10534090.png)
چکیده انگلیسی
A high-throughput screening method based on the competitive binding of a lumazine synthase inhibitor and riboflavin to the active site of Schizosaccharomyces pombe lumazine synthase was developed. This assay is sensitive, simple, and robust. During assay development, all of the known active inhibitors tested were positively identified. Preliminary high-throughput screening in 384-well format resulted in a Z factor of 0.7. The approach utilizes a thermodynamic assay to bypass the problems associated with the instabilities of both lumazine synthase substrates that complicate the use of a kinetic assay in a high-throughput format, and it removes the time element from the assay, thus simplifying the procedure.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 338, Issue 1, 1 March 2005, Pages 124-130
Journal: Analytical Biochemistry - Volume 338, Issue 1, 1 March 2005, Pages 124-130
نویسندگان
Jinhua Chen, Boris Illarionov, Adelbert Bacher, Markus Fischer, Ilka Haase, Gunda Georg, Qi-zhuang Ye, Zeqiang Ma, Mark Cushman,