کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10534095 | 961904 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel means of asymmetric linker attachment to DNA molecule by RecA-mediated multistranded DNA formation
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A novel means of asymmetric linker attachment to DNA molecule by RecA-mediated multistranded DNA formation A novel means of asymmetric linker attachment to DNA molecule by RecA-mediated multistranded DNA formation](/preview/png/10534095.png)
چکیده انگلیسی
We have developed a novel asymmetric linker attachment technology that utilizes multistranded DNA formation mediated by the RecA protein and Exonuclease I. Multistranded DNA can readily be formed at the terminus of double-stranded DNA by a complementary oligonucleotide in the presence of RecA and Exonuclease I. We have explored the possibility of applying this finding to the asymmetric attachment of linkers to specific DNA termini. We show that these unique properties of the terminal triple-stranded structure can be applied to directly clone specific DNA sequences.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 338, Issue 1, 1 March 2005, Pages 143-150
Journal: Analytical Biochemistry - Volume 338, Issue 1, 1 March 2005, Pages 143-150
نویسندگان
Yasushi Shigemori,