کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10536223 962046 2005 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of methotrexate, several pteridines, and creatinine in human urine, previous oxidation with potassium permanganate, using HPLC with photometric and fluorimetric serial detection
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Determination of methotrexate, several pteridines, and creatinine in human urine, previous oxidation with potassium permanganate, using HPLC with photometric and fluorimetric serial detection
چکیده انگلیسی
A novel HPLC method, using UV and fluorimetric serial detection, for the simultaneous determination of methotrexate (MTX), five disease marker pteridines, and the reference metabolic subproduct creatinine (CREA) in human urine was established. A previous oxidation process using 10−3 M KMnO4 (pH 5.0) and 35 min of oxidation time was necessary to transform the analytes in the highly fluorescent pteridinic rings. CREA was not affected by the oxidative medium. Using Tris-HCl/NaCl buffer solution (pH 6.6) as mobile phase, MTX and the assayed pteridines were monitored by fluorescence at λem = 444 nm and λex = 280 nm and creatinine was monitored by absorption measurements at λabs = 230 nm. All components were well resolved in approximately 7 min. Detection limits, according the criteria of Clayton and co-workers, were 10 ng ml−1 for MTX, less than 1 ng ml−1 for all of the pteridines, and 4 μg ml−1 for CREA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 346, Issue 2, 15 November 2005, Pages 201-209
نویسندگان
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