کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10536281 | 962056 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A new method of biosensing with 1 μl of Escherichia coli suspension using atomic force microscopy
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We developed a new method for detecting bacterial cells from 1-μl samples with atomic force microscopy (AFM). The use of a parafilm surface as a sample palette was effective for reacting small amounts of samples with an AFM probe. This was due to the parafilm's hydrophobic, semitransparent, and nonadhesive surface. In this way, all processes, such as the surface functionalization of a cantilever and the adhesion of Escherichia coli cells to a cantilever, were easily completed. In addition, we succeeded in detecting cell adsorption on the same AFM cantilever by both the drive mode and the thermal mode. The resonance frequency shift caused by cell adhesion was clearly detected by the two modes for the first time. Our data indicated the potential of applying AFM nanobiosensing to extremely small amounts of samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 345, Issue 1, 1 October 2005, Pages 116-121
Journal: Analytical Biochemistry - Volume 345, Issue 1, 1 October 2005, Pages 116-121
نویسندگان
Satoshi Tanaka, Hiroaki Sugasawa, Takashi Morii, Takao Okada, Masanori Abe, Noriaki Kato, Reiko Kuroda, Taishi Nasu, Masayuki Nagai, Kazuo Umemura,