کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10747083 | 1048887 | 2015 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Combination of keratins and alpha-smooth muscle actin distinguishes secretory coils from ducts of eccrine sweat glands
ترجمه فارسی عنوان
ترکیب کراتین و آستیگماتیک عضله آلفا، کوتیلهای سریالی را از کانالهای غدد عرق اکریئن تشخیص میدهند
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کلمات کلیدی
PBSDAPIalpha-SMA4′,6-diamidino-2-phenylindole - 4 '، 6-دیامیدینو-2-فنیلینولBSA - BSAbovine serum albumin - آلبومین سرم گاوAlpha-smooth muscle actin - آکتا عضله آلفا صافMyoepithelial cells - سلول های MyoepithelialEccrine sweat glands - غده عرق اکریینphosphate buffer saline - فسفات بافر شورDuct - مجراKeratins - کراتین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Eccrine sweat glands are comprised of secretory coils and ducts, which are distinct in morphology and function. To better understand the roles of the two parts in development, homeostasis, wound repair and regeneration of eccrine sweat glands, we must distinguish between them. In this study, the localization of keratins and alpha-SMA in human eccrine sweat glands was examined by immunofluorescence staining. Based on the differential localization of keratins and alpha-SMA in different cell types, four pairs of antibodies (K5/K7, K5/alpha-SMA, K14/K7 and K14/alpha-SMA) were used to differentiate secretory coils from ducts by double-immunofluorescence staining. Immunofluorescence staining showed that myoepithelial cells of secretory coils expressed K5, K14 and alpha-SMA, whereas secretory cells of secretory coils expressed K7, K8, K15, K18 and K19. Ductal cells expressed K5, K8, K14 and K19. Double-staining showed that the secretory coils were K5+/K7+, K5+/alpha-SMA+, K14+/K7+ and K14+/alpha-SMA+, whereas ducts were K5+/K7â, K5+/alpha-SMAâ, K14+/K7â and K14+/alpha-SMAâ. In conclusion, by combining use of keratins and alpha-SMA antibodies, secretory coils can be easily differentiated from ducts in morphology.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Histochemica - Volume 117, Issue 3, April 2015, Pages 275-278
Journal: Acta Histochemica - Volume 117, Issue 3, April 2015, Pages 275-278
نویسندگان
Haihong Li, Xiang Zhang, Shaopeng Zeng, Xuexue Li, Bingna Zhang, Lu Chen, Changmin Lin, Mingjun Zhang, Shijie Tang, Xiaobing Fu,