کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10747138 | 1048894 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Isolation and characterization of porcine circumvallate papillae cells
ترجمه فارسی عنوان
جداسازی و مشخص کردن خوک ها سلول های پاپیلوم را دفع می کند
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
کلسیم داخل سلولی خوک، فرهنگ اولیه، سلول های گیرنده طعم،
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Animal food intake is primarily controlled by appetite, which is affected by food quality, environment, and the management and status of animal health. Sensing of taste is mediated by taste receptor cells and is central to appetite. Taste receptor cells possess distinctive physiological characteristics that permit the recognition of various stimuli in foods. Thus, cultures of porcine circumvallate papillae cells provide a model for identification of the molecular and functional characteristics of taste receptor cells. In this study, we described the isolation and culture of porcine circumvallate papillae, using tissue explants and enzymatic digestion, and showed continuous viability and expression of pivotal taste marker proteins for more than 9 passages. In addition, cultured cells showed dramatic rises in intracellular calcium upon stimulation with several taste stimuli (sweet, umami, bitter, and fat). These cultures of porcine taste receptor cells provide a useful model for assessing taste preferences of pigs and may elucidate interactions between various taste stimuli.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Histochemica - Volume 116, Issue 8, October 2014, Pages 1313-1322
Journal: Acta Histochemica - Volume 116, Issue 8, October 2014, Pages 1313-1322
نویسندگان
Zhi-Qi Zhang, Gang Shu, Xiao-Tong Zhu, Li-Na Wang, Qiang Fu, Lian-Jie Hou, Song-Bo Wang, Ping Gao, Qian-Yun Xi, Yong-Liang Zhang, Lin Yu, Ji-Rong Lv, Qing-Yan Jiang,