کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10747149 | 1048894 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Localization of Na+-K+-ATPase α/β, Na+-K+-2Cl-cotransporter 1 and aquaporin-5 in human eccrine sweat glands
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کلمات کلیدی
ABCPBSAquaporin-5AQP53′-diaminobenzidineHRPNKCC1BSA - BSACl− - Cl-Na+ - Na +bovine serum albumin - آلبومین سرم گاوImmunohistochemistry - ایمونوهیستوشیمیSodium - سدیم Myoepithelial cells - سلول های MyoepithelialEccrine sweat gland - غده عرق اکریینApical membrane - غشای اپیکیbasement membrane - غشای پایهLumen - لومنPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریPotassium - پتاسیمHorseradish peroxidase - پراکسیداز هوررادیشavidin–biotin complex - پیچیده avidin-بیوتینducts - کانال هاChloride - کلرید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
In order to evaluate the function of the repaired or regenerated eccrine sweat glands, we must first localize the proteins involved in sweat secretion and absorption in normal human eccrine sweat glands. In our studies, the cellular localization of Na+-K+-ATPase α/β, Na+-K+-2Cl-cotransporter 1 (NKCC1) and aquaporin-5 (AQP5) in eccrine sweat glands were detected by immunoperoxidase labeling. The results showed that Na+-K+-ATPase α was immunolocalized in the cell membrane of the basal layer and suprabasal layer cells of the epidermis, the basolateral membrane of the secretory coils, and the cell membrane of the outer cells and the basolateral membrane of the luminal cells of the ducts. The localization of Na+-K+-ATPase β in the secretory coils was the same as Na+-K+-ATPase α, but Na+-K+-ATPase β labeling was absent in the straight ducts and epidermis. NKCC1 labeling was seen only in the basolateral membrane of the secretory coils. AQP5 was strongly localized in the apical membrane and weakly localized in the cytoplasm of secretory epithelial cells. The different distribution of these proteins in eccrine sweat glands was related to their functions in sweat secretion and absorption.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Histochemica - Volume 116, Issue 8, October 2014, Pages 1374-1381
Journal: Acta Histochemica - Volume 116, Issue 8, October 2014, Pages 1374-1381
نویسندگان
Mingjun Zhang, Shaopeng Zeng, Lei Zhang, Haihong Li, Lu Chen, Xiang Zhang, Xuexue Li, Changmin Lin, Shenyou Shu, Sitian Xie, Yunpu He, Xiaoyan Mao, Lihong Peng, Lungang Shi, Lvjun Yang, Shijie Tang, Xiaobing Fu,