کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10747228 | 1048914 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In situ study of the gelatinase activity in demineralized dentin from rat molar teeth
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: In situ study of the gelatinase activity in demineralized dentin from rat molar teeth In situ study of the gelatinase activity in demineralized dentin from rat molar teeth](/preview/png/10747228.png)
چکیده انگلیسی
Matrix metalloproteinases (MMPs) in dentin are believed to participate in various physiological and pathological events in coronal dentin, but their exact source and location is not clear. The purpose of this study was to evaluate the activity of gelatinases in decalcified rat molars crowns by in situ zymography. Hemi-mandibles of five male Wistar rats were fixed in paraformaldehyde, decalcified in EDTA and glycerol solution and embedded in paraffin. Sections from the region of molar teeth were incubated with or without DQ gelatin in 50 mM Tris-CaCl2 at 37 °C for 2 h and observed by means of confocal microscopy. Gelatinolytic activity was observed throughout the coronal dentin with varying intensities in different locations. High gelatinase activity was observed in the dentinal tubules, dentin-enamel junction (DEJ) and predentin, and it was weaker and less uniform in the intertubular dentin. This study shows that the location of gelatinase and relative activity can be detected by means of in situ zymography and confocal microcopy, and this methodology may provide a useful tool in studies on the role of gelatinases in tooth development, maturation and in pathological conditions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Histochemica - Volume 115, Issue 3, April 2013, Pages 245-251
Journal: Acta Histochemica - Volume 115, Issue 3, April 2013, Pages 245-251
نویسندگان
Juliana Isabelita Cyrino Pessoa, Gustavo Narvaes Guimarães, Naiana Viana Viola, Wander José da Silva, Ana Paula de Souza, Leo Tjäderhane, Sergio Roberto Line, Marcelo Rocha Marques,