کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10819110 | 1060352 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A comparison of the counteracting effects of glycine betaine and TMAO on the activity of RNase A in aqueous urea solution
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A comparison of the counteracting effects of glycine betaine and TMAO on the activity of RNase A in aqueous urea solution A comparison of the counteracting effects of glycine betaine and TMAO on the activity of RNase A in aqueous urea solution](/preview/png/10819110.png)
چکیده انگلیسی
Trimethylamine-N-oxide (TMAO) and glycine betaine are counteracting osmolytes found in cellular systems under osmotic stress, often in association with high urea concentrations. TMAO is a characteristic component of cartilaginous fish and marine molluscs, while glycine betaine is more widely distributed, occurring in plants, bacteria and the mammalian kidney. As part of a project to explain and understand the action of these methylamines, the RNase A-catalysed degradation of polyuridylic acid in the presence of urea and various osmolytes (0-1.0 M) was studied using 31P Nuclear Magnetic Resonance spectroscopy. The decrease in reaction rate induced by urea could be fully recovered with 1 molar equivalent of trimethylamine-N-oxide or 1.4 molar equivalents of glycine betaine. These results indicate that the modification of RNase A activity induced by urea is not associated with gross irreversible structural changes and that both glycine betaine and trimethylamine-N-oxide have kinetically detectable counteracting effects.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology - Volume 141, Issue 1, May 2005, Pages 22-28
Journal: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology - Volume 141, Issue 1, May 2005, Pages 22-28
نویسندگان
L.M. Samuelsson, J.J. Bedford, R.A.J. Smith, J.P. Leader,