کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10819257 | 1060371 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Differential effects of temperature and maturity stage on hepatic estrogen receptor characteristics of Atlantic salmon
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Differential effects of temperature and maturity stage on hepatic estrogen receptor characteristics of Atlantic salmon Differential effects of temperature and maturity stage on hepatic estrogen receptor characteristics of Atlantic salmon](/preview/png/10819257.png)
چکیده انگلیسی
In order to determine if elevated temperature during vitellogenesis had a detrimental effect on hepatic estrogen receptors of Atlantic salmon (Salmo salar), 3H-estradiol saturation binding analysis, using one- and two-site binding models, was carried out on extracts of hepatic cytosols from fish held at 14, 18 or 22 °C over the austral period of peak vitellogenesis (February to April). With one-site binding analysis, no temperature related difference in either receptor affinity (Kd) or number (Bmax) was found at each sampling point, but there was an apparent decrease in both affinity and number at each temperature over the period of the study. However, some analyses, notably at 22 °C during February, were best described using a two-site binding model. At this temperature and time, there was a clear separation of binding affinity into high and low components (Kd=0.67±S.E. 0.05 and 20±S.E. 5.6 nM, respectively) (n=4), which suggests that February was a critical time of temperature related hepatic sensitivity to estrogen. These results support those of other studies where we found that February was also a sensitive time with respect to temperature impairment of in vitro follicular estrogen synthesis, and the greatest period of in vivo temperature sensitivity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology - Volume 140, Issue 3, March 2005, Pages 377-383
Journal: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology - Volume 140, Issue 3, March 2005, Pages 377-383
نویسندگان
M. Watts, N.W. Pankhurst, H.R. King, D.P. Geraghty,