Gonadotropin-inhibitory hormone (GnIH) and its receptor in the female pig: cDNA cloning, expression in tissues and expression pattern in the reproductive axis during the estrous cycle

► We cloned pig GnIH cDNA 442 bp and GPR147 cDNA 1003 bp. ► Tissue expression revealed that GnIH and GPR147 was mainly expressed in the brain. ► Fluctuations of GnIH and GPR147 expression were found during the pig estrous cycle. ► GnIH and GPR147 were mainly localized in the luteal cells, granulosa, and theca cells.