Neuromedin B stimulates proliferation of mouse chondrogenic cell line ATDC5

► NMB and its receptor (NMB-R) were expressed in mouse chondrogenic cell line, ATDC5. ► Differentiation of ATDC5 cells transiently increased gene expression of NMB on day 4. ► NMB-R expression was decreased on days 7 and 14 in differentiation of ATDC5 cells. ► NMB significantly induced ATDC5 proliferation, but did not affect its differentiation.