Effect of antimicrobial peptides on ATPase activity and proton pumping in plasma membrane vesicles obtained from mycobacteria

► Well-known AMPs shows an antimycobacterial activity low and variable. ► The AMPs activity of the designed MIAP is 3-fold higher than the native peptide. ► AMPs partially inhibits the ATPase activity of mycobacterial plasma membrane vesicles. ► MIAP abolishes the F1F0-ATPase activity involved in H+ pumping across M. tuberculosis.