In vitro and in vivo studies on the antioxidant activity of fish peptide isolated from the croaker (Otolithes ruber) muscle protein hydrolysate

► Simple procedure was used to purify the peptide. ► Peptides had reducing, metal chelating ability besides scavenging radicals. ► It is successful in inhibiting the lipid peroxidation and DNA damage. ► Its ability to enhance in vivo antioxidant enzymes was proved. ► Peptide acted as a shield against ethanol induced free radicals in Wistar rats.