Neuropeptide FF activates ERK and NF kappa B signal pathways in differentiated SH-SY5Y cells

► We first report a differentiated SH-SY5Y cell line dSH-SY5Y endogenously expresses functional hNPFF2 receptor. ► NPFF activates the ERK signal pathway in a PKA- and nitric oxide synthase-dependent manner. ► NPFF stimulates the NF kappa B signal pathway in an ERK-independent way.