Intracerebroventricular administration of neuronostatin delays gastric emptying and gastrointestinal transit in mice

► Intracerebroventricular administration of neuronostatin delayed gastric emptying and gastrointestinal transit in mice. ► The gastrointestinal effect elicited by neuronostatin was significantly reversed by melanocortin 3/4 receptor antagonist SHU9119 or classical opioid receptor antagonist naloxone. ► Intracerebroventricular administration of non-amidated neuronostatin had no influence on gastric emptying and gastrointestinal transit.