Identification and characterization of antimicrobial peptides from skin of Amolops ricketti (Anura: Ranidae)

► cDNAs encoding five novel antimicrobial peptide precursors were cloned from skin cDNA library of Amolops ricketti. ► They are the first antimicrobial peptides from A. ricketti.► The actual presence and characterization of mature antimicrobial peptides was confirmed by two methods RP-HPLC and LC-MS/MS-based proteomics approach. ► We use the proteomic method in 4 different ways to prepare the sample for peptides identification. ► Incompletely digestion of protein can markedly increase peptide numbers of identification.