کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10836176 | 1066408 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Synthesis and biological properties of chimeric interferon-α2b peptides
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
We have previously reported the antiproliferative activity of synthetic sequences 29-35 and 122-139 of the interferon-α2b (IFN-α2b), both probably representing a common receptor recognition domain. In the search of new peptidic agonists, we designed and synthesized the linear peptide (Gly)2-122-137-Gly138-Gly29-30-35-(Gly)2, in which Gly residues replaced the 138 and 29 Cys bound through a disulfide bridge in the native cytokine. Additionally, a cyclic analog was obtained by reaction of the N- and C-terminal ends of the linear fragment. Thus, the distance that separates residues 122 and 35 in the crystalline structure of the IFN-α2b was maintained through a (Gly)4 bridge. When the influence of chimeric peptides on the proliferation of WISH cells was studied, it was shown that both derivatives significantly diminished cell growth. A more evident inhibitory effect on 125I-IFN-α2b binding to WISH cell-membrane receptors was observed for both peptides. Results indicated that chimeric IFN-α2b peptides behaved as partial agonists of the IFN-α2b molecule and may be of interest for drug design purposes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Peptides - Volume 26, Issue 7, July 2005, Pages 1144-1149
Journal: Peptides - Volume 26, Issue 7, July 2005, Pages 1144-1149
نویسندگان
Clara Peña, Viviana C. Blank, Verónica J. Marino, Leonor P. Roguin,