کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10837016 | 1066459 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Involvement of cyclooxygenase-dependent pathway in contraction of isolated ileum by urotensin II
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We previously reported that urotensin II induced biphasic (brief- and long-lasting) contractions and the brief contraction was mediated by acetylcholine release from ganglionic cholinergic neurons in a segment of guinea-pig ileum. In the present work, we studied the mechanism contributing to long-lasting contractions induced by urotensin II. Treatment with 0.1 μM tetrodotoxin, 300 nM Ï-conotoxin GVIA (an inhibitor of N-type Ca2+ channels) and 10 μM indomethacin (an inhibitor of cyclooxygenases) markedly inhibited 100 nM urotensin II-induced long-lasting contractions. The addition of 1 μM prostaglandin F2α (PGF2α) caused a limited brief contraction following long-lasting contraction, while 1 μM PGE2 induced marked biphasic contractions. Treatment with neurotoxins inhibited the long-lasting contractions induced by PGF2α and PGE2 without changing the PGE2-induced brief contractions. Treatment with 1 μM atropine markedly inhibited the urotensin II- and PGF2α-induced long-lasting contractions, but was less effective on the PGE2 responses. Treatment with a phospholipase A2 inhibitor decreased the urotensin II-induced contractions. These findings suggest that urotensin II induces, at least partially, long-lasting contractions via PG-sensitive cholinergic neurons and muscarinic acetylcholine receptors in the ileum.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Peptides - Volume 26, Issue 2, February 2005, Pages 323-329
Journal: Peptides - Volume 26, Issue 2, February 2005, Pages 323-329
نویسندگان
Syunji Horie, Yuumi Tsurumaki, Akiyoshi Someya, Tetsuya Hirabayashi, Takeshi Saito, Yasunobu Okuma, Yasuyuki Nomura, Toshihiko Murayama,