LC-MS/MS as a tool for identification of bioactive compounds in marine sponge Spongosorites halichondriodes (Dendy 1905)

► LC-MS/MS technique used for identification and characterization of the compounds in marine sponge S. halichondriodes. ► The bioactive compounds confirmed by the fragmentation pattern, depending on m/z and intensity of the ions. ► The six different compounds belong to chemical class like sterol esters, nucleosides, triterpenoid and isocoumarin class. ► The compounds found in crude extracts are sterol esters, citrinolactone A, glycyrrhetinic acid and inosine.