Investigation of extraction and analysis techniques for Lyngbya wollei derived Paralytic Shellfish Toxins

► A Lyngbya wollei dominated mat sample was extracted in triplicate with 2 different acids. ► Acids at varying concentrations were compared to assess extraction efficiency and toxin profile. ► Analysis with pre-chromatographic LC/FL was compared to LC/MS analysis. ► 0.1 M HCl resulted in hydrolysis of L. wollei Toxins 1-6 to more toxic variants. ► Analysis with LC/FL overestimated toxicity when compared to LC/MS analysis for L. wollei derived PSTs.