Investigating the chemical profile of regenerated scorpion (Parabuthus transvaalicus) venom in relation to metabolic cost and toxicity

► Milked scorpions had a higher metabolic rate than unmilked scorpions. ► Parabuthus transvaalicus venom components seem to be regenerated asynchronously. ► Peptides associated with prevenom seem to be regenerated before venom peptides. ► Lethality bioassay showed that venom toxicity returned to normal within 4 days. ► Complexity of venom is associated with higher metabolic cost of venom regeneration.