Determination of swainsonine in the endophytic Undifilum fungi by high-performance liquid chromatography with evaporative light-scattering detector

► For the first time, swainsonine was separated well on the HILIC column. ► For the first time, swainsonine was detected by ELSD. ► A HPLC-ELSD method has been developed for the quantification of swainsonine. ► The method was applied to quantitative analysis of swainsonine in fungi samples.