Isolation and characterization of moojenin, an acid-active, anticoagulant metalloproteinase from Bothrops moojeni venom

► We describe the purification/characterization of a metalloproteinase from Bothrops moojeni. ► The enzyme was originated from the autolysis processes of a PIIIb class of SVMPS. ► The enzyme displays coagulant and fibrinogenolytic activities. ► The enzyme is active at acidic pH but not in pHs ranging from neutral to basic.