Functional characterization of Kunitz-type protease inhibitor Pr-mulgins identified from New Guinean Pseudechis australis

► We identified three Kunitz-type protease inhibitors named Pr-mulgins 1, 2, and 3. ► Pr-mulgin 1 specifically inhibited MMP2 activity. ► Pr-mulgins 2 and 3 showed potent inhibition to some serine proteases. ► Pr-mulgins 1, 2, and 3 had essentially no effect on K+ channels (Shaker and Kv 1.1).