کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10880765 | 1076995 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Lys-64 of the A chain is involved in the enzymatic activity and neurotoxic effect of β-bungarotoxin
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Lys-64 of the A chain is involved in the enzymatic activity and neurotoxic effect of β-bungarotoxin Lys-64 of the A chain is involved in the enzymatic activity and neurotoxic effect of β-bungarotoxin](/preview/png/10880765.png)
چکیده انگلیسی
Two β-bungarotoxin isotoxins BM12 and BM13 were isolated from Bungarus multicinctus (Taiwan banded krait) venom by sequential chromatography on ion-exchange and reverse phase columns. The two toxins have the same A chain, but different B chains. Different phospholipase A2 activity and different potencies in inhibiting the spontaneous enhancement of spontaneous synaptic current frequency and muscle contraction were observed for BM12 and BM13. Nevertheless, modification of Lys-64 in the A chain of BM12 and BM13 similarly reduced in their phospholipase A2 activity and toxicity. The modified derivatives retained their affinity with Ca2+ and their conformation as deduced by CD. These results suggest that Lys-64 of the A chain is involved in the phospholipase A2 activity and in the neurotoxic effect of β-bungarotoxin.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicon - Volume 45, Issue 2, February 2005, Pages 179-185
Journal: Toxicon - Volume 45, Issue 2, February 2005, Pages 179-185
نویسندگان
Long-Sen Chang, Yuan-Ping Chu, Yun-Ching Cheng, Jau-Cheng Liou, Chen-Chung Yang,