کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10881033 1077005 2005 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Isolation and molecular cloning of novel peptide toxins from the sea anemone Antheopsis maculata
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
Isolation and molecular cloning of novel peptide toxins from the sea anemone Antheopsis maculata
چکیده انگلیسی
Three peptide toxins (Am I-III) with crab toxicity were isolated from the sea anemone Anthopleura maculata by gel filtration and reverse-phase HPLC. Am I was weakly lethal to crabs (LD50 830 μg/kg) and Am III was potently lethal (LD50 70 μg/kg), while Am II was only paralytic (ED50 420 μg/kg). The complete amino acid sequences of the three toxins were determined by cDNA cloning based on 3′-Race and 5′-Race. Although Am III (47 residues) is an analogue of the well-known type 1 sea anemone sodium channel toxins, both Am I (27 residues) and II (46 residues) are structurally novel peptide toxins. Am I is a new toxin having no sequence homologies with any toxins. Am II shares 28-39% identity with the recently characterized sea anemone toxins inhibiting specialized ion channels, BDS-I and II from Anemonia sulcata and APETx1 and 2 from Anthopleura elegantissima. The precursor proteins of the three toxins are commonly composed of a signal peptide, a propart with a pair of basic residues (Lys-Arg) at the end and the remaining portion. Very interestingly, the Am I precursor protein contains as many as six copies of Am I.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicon - Volume 45, Issue 1, January 2005, Pages 33-41
نویسندگان
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