کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10891275 | 1081998 | 2013 | 17 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Endothelial cells provide a niche for placental hematopoietic stem/progenitor cell expansion through broad transcriptomic modification
ترجمه فارسی عنوان
سلولهای اندوتلیال، توالی را برای گسترش سلول های ساقه مغز / ژن ژنتیکی جفتی به وسیله اصلاح گسترده ترانسکتومیک فراهم می کنند
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
چکیده انگلیسی
Umbilical cord blood (UCB) is an attractive source of hematopoietic stem cells (HSCs). However, the number of HSCs in UCB is limited, and attempts to amplify them in vitro remain inefficient. Several publications have documented amplification of hematopoietic stem/progenitor cells (HSPCs) on endothelial or mesenchymal cells, but the lack of homogeneity in culture conditions and HSC definition impairs direct comparison of these results. We investigated the ability of different feeder layers, mesenchymal progenitors (MPs) and endothelial cells (ECs), to amplify hematopoietic stem/progenitor cells. Placental derived HSPCs (defined as LinâCD45â/dimCD34+CD38âCD90+) were maintained on confluent feeder layers and the number of cells and their marker expression were monitored over 21Â days. Although both types of feeder layers supported hematopoietic expansion, only endothelial cells triggered amplification of LinâCD45â/dimCD34+CD38âCD90+ cells, which peaked at 14Â days. The amplified cells differentiated into all cell lineages, as attested by in vitro colony-forming assays, and were capable of engraftment and multi-lineage differentiation in sub-lethally irradiated mice. Mesenchymal progenitors promoted amplification of CD38+ cells, previously defined as precursors with more limited differentiation potential. A competitive assay demonstrated that hematopoietic stem/progenitor cells had a preference for interacting with endothelial cells in vitro. Cytokine and transcriptomic analysis of both feeder cell types identified differences in gene expression that correlated with propensity of ECs and MPs to support hematopoietic cell amplification and differentiation respectively. Finally, we used RNA sequencing of endothelial cells and HSPCs to uncover relevant networks illustrating the complex interaction between endothelial cells and HSPCs leading to stem/progenitor cell expansion.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Stem Cell Research - Volume 11, Issue 3, November 2013, Pages 1074-1090
Journal: Stem Cell Research - Volume 11, Issue 3, November 2013, Pages 1074-1090
نویسندگان
Christophe M. Raynaud, Jason M. Butler, Najeeb M. Halabi, Faizzan S. Ahmad, Badereldeen Ahmed, Shahin Rafii, Arash Rafii,