کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10891281 1081998 2013 15 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
PKHhigh cells within clonal human nephrospheres provide a purified adult renal stem cell population
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
PKHhigh cells within clonal human nephrospheres provide a purified adult renal stem cell population
چکیده انگلیسی
The existence and identification of adult renal stem cells is a controversial issue. In this study, renal stem cells were identified from cultures of clonal human nephrospheres. The cultured nephrospheres exhibited the activation of stem cell pathways and contained cells at different levels of maturation. In each nephrosphere the presence of 1.12-1.25 cells mirroring stem cell properties was calculated. The nephrosphere cells were able to generate three-dimensional tubular structures in 3D cultures and in vivo. In clonal human nephrospheres a PKHhigh phenotype was isolated using PKH26 epifluorescence, which can identify quiescent cells within the nephrospheres. The PKHhigh cells, capable of self-renewal and of generating a differentiated epithelial, endothelial and podocytic progeny, can also survive in vivo maintaining the undifferentiated status. The PKHhigh status, together with a CD133+/CD24− phenotype, identified a homogeneous cell population displaying in vitro self-renewal and multipotency capacity. The resident adult renal stem cell population isolated from nephrospheres can be used for the study of mechanisms that regulate self-renewal and differentiation in adult renal tissue as well as in renal pathological conditions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Stem Cell Research - Volume 11, Issue 3, November 2013, Pages 1163-1177
نویسندگان
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