Computer-Aided 2D and 3D quantification of human stem cell fate from in vitro samples using Volocity high performance image analysis software

► Manual image analysis of stem cell fate has low internal and external validity. ► Image analysis software aids in stem cell classification and quantification. ► Optimization of confocal settings improves efficiency and accuracy of fate analysis. ► Human operator validation of software analysis is recommended for better accuracy. ► Human operator validated image analysis is 2-fold faster than manual counts.